《植物生理学报》 2018, 54(6): 943-950

甘蔗杆状病毒及其启动子功能的研究进展

孙生仁¹, 张慧丽¹, 吴小斌², 高三基^1,*

¹福建农林大学国家甘蔗工程技术研究中心, 福州350002; ²广东省生物工程研究所(广州甘蔗糖业研究所), 广州510316

通信作者：高三基；E-mail: gaosanji@yahoo.com

摘　要：

甘蔗杆状病毒(SCBV)是侵染甘蔗的重要病原物之一, 属于花椰菜花叶病毒科(Caulimoviridae)杆状DNA病毒属(Badnavirus)。该病害在多数种植甘蔗的国家和地区普遍发生, 可导致甘蔗品质和产量下降, 对甘蔗产业构成潜在威胁。SCBV基因组为环状双链DNA, 长度为7.3~8.0 kb。不同地理来源的SCBV分离物遗传多样性丰富, 全球至少存在18种系统进化组群或基因型(SCBV-A~R), 其中, 来自摩洛哥的SCBMOV、澳大利亚的SCBIMV及瓜德罗普岛的SCBGAV和SCBGDV被国际病毒分类委员会(ICTV)认定为杆状DNA病毒属下的4个不同病毒种。SCBV基因组编码3个开放阅读框(ORF), 其中ORF3的3′端和ORF1的5′端之间基因间隔区域为病毒启动子区域。本文主要综述了SCBV生物学和基因组特性、遗传多样性、检测方法、病毒启动子的功能及应用等方面的最新研究进展, 旨在为SCBV的进一步研究提供参考。

关键词：杆状DNA病毒; 甘蔗杆状病毒; 遗传多样性; 基因组结构; 病毒启动子

收稿：2018-03-21   修定：2018-05-28

资助：国家现代农业产业技术体系(糖料)建设专项(CARS-170302)和福建省科技重大专项(2015NZ0002-2)。

Research advances in sugarcane bacilliform virus and its encoded promoter

SUN Sheng-Ren¹, ZHANG Hui-Li¹, WU Xiao-Bin², GAO San-Ji^1,*

¹National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China; ²Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou 510316, China

Corresponding author: GAO San-Ji; E-mail: gaosanji@yahoo.com

Abstract:

Sugarcane bacilliform virus (SCBV; genus Badnavirus, family Caulimoviridae), an important pathogen infecting sugarcane, is prevalent throughout most sugarcane-producing regions and countries. SCBV causes yield losses and decreases juice quality of sugarcane, posing a potential threat to the sugarcane industry. The genome is circular, double-stranded, and 7.3–8.0 kilobase pairs in length. The genomic sequences of SCBV isolates are highly variable and 18 SCBV genotypes/strains (from SCBV-A to -R) have been reported worldwide. The four species, Sugarcane bacilliform MO virus (SCBMOV), Sugarcane bacilliform IM virus (SCBIMV), Sugarcane bacilliform Guadeloupe A virus (SCBGAV) and Sugarcane bacilliform Guadeloupe D virus (SCBGDV) were assigned to the genus Badnavirus by the International Committee on Taxonomy of Viruses (ICTV). The SCBV genome contains three open reading frames (ORFs) and a putative promoter region that is located at the intergenic region (IGR) between the 3′ end of ORF3 and the 5′ end of ORF1. Research advances in SCBV and its putative promoter functions are reviewed including aspects of viral biology characteristics, methods of detection, genetic diversity, genomic structure and viral promoter. This review will provide valuable insight into further research of SCBV.

Key words: Badnavirus; sugarcane bacilliform virus; genetic diversity; genomic construct; viral promoter